MFSD10 Polyclonal antibody

• 

  WB analysis of mouse heart using 11518-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11518-1-AP (MFSD10 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human stomach using 11518-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11518-1-AP (MFSD10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MFSD10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11518-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TETRAN, Tetracycline transporter-like protein, Major facilitator superfamily domain-containing protein 10

---

Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11518-1-AP targets MFSD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFSD10 fusion protein Ag2080
• Full Name: major facilitator superfamily domain containing 10
• Calculated Molecular Weight: 455 aa, 48 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: BC014979
• Gene Symbol: MFSD10
• Gene ID (NCBI): 10227
• RRID: AB_2877773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14728
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.; Authors - Mehdi Eshraghi
mouse	IHC	Biogerontology; Histone lysine methyltransferases and their specific methylation marks show significant changes in mouse testes from young to older ages; Authors - Yesim Bilmez
		J Assist Reprod Genet; The DNA double-strand break repair proteins γH2AX, RAD51, BRCA1, RPA70, KU80, and XRCC4 exhibit follicle-specific expression differences in the postnatal mouse ovaries from early to older ages; Authors - Gunel Talibova