MGAT1 Polyclonal antibody

MGAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15103-1-AP

Synonyms

GlcNAc-T I, GLCNAC TI, GlcNAc T I, GGNT1, EC:2.4.1.101

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Validation Data Gallery

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  WB analysis of Y79 using 15103-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15103-1-AP (MGAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15103-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15103-1-AP (MGAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15103-1-AP (MGAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse liver using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15103-1-AP

  Immunofluorescent analysis of Hela cells, using MGAT1 antibody 15103-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Y79 cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue, mouse heart tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15103-1-AP targets MGAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGAT1 fusion protein Ag7446
• Full Name: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC003575
• Gene Symbol: MGAT1
• Gene ID (NCBI): 4245
• RRID: AB_2297618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Arthritis Rheum; Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage.; Authors - Urita Atsushi A
mouse	WB	FASEB J; Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.; Authors - Andrew R Ednie
human, rat	WB	Schizophr Res; Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia.; Authors - Jordyn M Kippe
mouse	WB	Liver Transpl; Monoacylglycerol Acyltransferase 1 Knockdown Exacerbates Hepatic Ischemia/Reperfusion Injury in Mice With Hepatic Steatosis.; Authors - Kim H H Liss
rat	WB,IF	Sci Rep; Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats.; Authors - Kaimei Lu
human	WB	Biochemistry; N-linked glycosylation dependent and independent mechanisms regulating CTRP12 cleavage, secretion, and stability.; Authors - Ashley N Stewart