MGAT2 Polyclonal antibody

MGAT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15772-1-AP

Synonyms

CDGS2, GlcNAc T II, GLCNACTII, GNT II, GNT2, MGAT2

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Validation Data Gallery

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  WB analysis of human liver using 15772-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15772-1-AP (MGAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15772-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15772-1-AP (MGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 15772-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15772-1-AP (MGAT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15772-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15772-1-AP (MGAT2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human liver tissue, mouse heart tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15772-1-AP targets MGAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGAT2 fusion protein Ag8423
• Full Name: mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
• Calculated Molecular Weight: 447 aa, 52 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC006390
• Gene Symbol: MGAT2
• Gene ID (NCBI): 4247
• RRID: AB_2142907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.