MGAT3 Polyclonal antibody

• Featured Product
• KD/KO Validated

MGAT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17869-1-AP

Synonyms

GGNT3, GlcNAc T III, GNT III, GNT3, MGAT3

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Validation Data Gallery

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  WB analysis of COLO 320 using 17869-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 17869-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 17869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, Caco-2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

17869-1-AP targets MGAT3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGAT3 fusion protein Ag12352
• Full Name: mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• Calculated Molecular Weight: 533 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC075026
• Gene Symbol: MGAT3
• Gene ID (NCBI): 4248
• RRID: AB_10597697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In the small intestine, ~75% of triacylglycerol (TG) synthesis occurs via the monoacylglycerol acyltransferase (MGAT) pathway.Three MGAT isoforms (MGAT1, MGAT2 and MGAT3) have been identifed based on their extensive homology to DGAT2. MGAT1 is primarily expressed in the adipose tissue, stomach and kidney but not in the small intestine. MGAT2 and MGAT3 are most highly expressed in the small intestine, suggesting that these two MGAT forms are responsible for the resynthesis of dietary TG that is packaged into chylomicrons.

Publications

Species	Application	Title
human,mouse	WB,IHC	Theranostics; MGAT3-mediated glycosylation of tetraspanin CD82 at asparagine 157 suppresses ovarian cancer metastasis by inhibiting the integrin signaling pathway.; Authors - Jun Li ; KD Validated
	WB	J Biol Chem; LacdiNAc synthase B4GALNT3 has a unique PA14 domain and suppresses N-glycan capping; Authors - Yuko Tokoro
human	IHC	Theranostics; Bisecting GlcNAc modification reverses the chemoresistance via attenuating the function of P-gp; Authors - Zengqi Tan
		Neuropharmacology; DHEC mesylate attenuates pathologies and aberrant bisecting N-glycosylation in Alzheimer's disease models; Authors - Yue Wang
human,mouse	WB,IHC,IF	Pharmacol Res; Targeting aberrant glycosylation to modulate microglial response and improve cognition in models of Alzheimer's disease; Authors - Yue Wang