Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17841-1-AP targets MGAT4C in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12201 Product name: Recombinant human MGAT4C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 127-478 aa of BC064141 Sequence: SSYEELKEISVVVHLADFNSSWRDAMVQDITQKFAHHIIAGRLMVIHAPEEYYPILDGLKRNYNDPEDRVKFRSKQNVDYAFLLNFCANTSDYYVMLEDDVRCSKNFLTAIKKVIASLEGTYWVTLEFSKLGYIGKLYHSHDLPRLAHFLLMFYQEMPCDWLLTHFRGLLAQKNVIRFKPSLFQHMGYYSSYKGTENKLKDDDFEEESFDIPDNPPASLYTNMNVFENYEASKAYSSVDEYFWGKPPSTGDVFVIVFENPIIIKKIKVNTGTEDRQNDILHHGALDVGENVMPSKQRRQCSSYLRLGEFKNGNFEMSGVNQKIPFDIHCMRIYVTKTQKEWLIIRSISIWTS Predict reactive species |
| Full Name | mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative) |
| Calculated Molecular Weight | 478 aa, 56 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC064141 |
| Gene Symbol | MGAT4C |
| Gene ID (NCBI) | 25834 |
| RRID | AB_2143048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MGAT4C is predicted to be a glycosyltransferase that catalyzes a GlcNAc β1-4 linkage to the core mannose residues of N-glycans, resulting in N-glycans with tri- or tetraantennary structures.Recently MGAT4C has been found to get involved in processing complex N-glycan structures on CD133 and CD133 has been identified as a substrate of MGAT4C. Given to the fact that CD133 is widely used as a marker of numerous stem cell and cancer stem cell types, MGAT4C may have an important role in certain cancers. (PMID: 21937449)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MGAT4C antibody 17841-1-AP | Download protocol |
| WB protocol for MGAT4C antibody 17841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MGAT4C polyclonal antibody (Cat# 17841-1-AP) has 4 citations published in Developmental Cell, Clinical and Translational Medicine, Journal of Biological Chemistry, and Biomedical Research International. The associated research fields include colitis and wound healing, prostate cancer and lipid metabolism, CD133 N-glycosylation and stem cell biology, and ischemic stroke biomarker discovery.
| Species | Application | Title |
|---|---|---|
Dev Cell Wound-healing plasticity enables clonal expansion of founder progenitor cells in colitis | ||
Clin Transl Med Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1. | ||
J Biol Chem CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope.
| ||
Biomed Res Int Mendelian Randomization and Transcriptome Analysis Identify Ischemic Stroke Biomarkers With Putative Relevance to Cerebrospinal Fluid. |
