MGMT Recombinant antibody, PBS Only

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  WB analysis using 86115-5-RR (same clone as 86115-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 89933-4-RR (MGMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 86115-5-RR (same clone as 86115-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MGMT antibody (89933-4-RR, Clone: 243130G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

MGMT Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86115-5-PBS

Clone No.243130G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

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Product Information

86115-5-PBS targets MGMT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MGMT fusion protein Ag9996
• Full Name: O-6-methylguanine-DNA methyltransferase
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC000824
• Gene Symbol: MGMT
• Gene ID (NCBI): 4255
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16455
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment (PMID:23065697). MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine (PMID: 17482892). The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea (PMID: 10767620).