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  • KD/KO Validated

MGP Polyclonal antibody

MGP Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10734-1-AP

Synonyms

GIG36, matrix Gla protein, MGLAP, MGP, NTI



Tested Applications

Positive IHC detected inhuman colon cancer tissue, human small intestine tissue, human breast cancer tissue, human lymphoma tissue, human breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10734-1-AP targets MGP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MGP fusion protein Ag1091
Full Name matrix Gla protein
Calculated molecular weight 103 aa, 13 kDa
GenBank accession numberBC005272
Gene symbol MGP
Gene ID (NCBI) 4256
RRIDAB_2297660
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Matrix Gla protein (MGP), a vitamin K-dependent protein, is recognized as a calcification inhibitor in vascular tissue. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.

Protocols

Product Specific Protocols
IHC protocol for MGP antibody 10734-1-APDownload protocol
IF protocol for MGP antibody 10734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Circulation

Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and atherosclerosis.

Authors - Chang Kyunghwa K
mouseWB

Nat Commun

Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.

Authors - Mehdi Eshraghi
humanIHC

Arthritis Rheum

Juvenile dermatomyositis calcifications selectively displayed markers of bone formation.

Authors - Urganus Annette L AL
ratWB

Aging (Albany NY)

Intermedin1-53 attenuates aging-associated vascular calcification in rats by upregulating sirtuin 1.

Authors - Yao Chen
humanWB

FASEB J

2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes injury-induced vascular neointima formation in mice.

Authors - Shumin Guo
humanWB

Int J Obes (Lond)

Using gene expression to predict the secretome of differentiating human preadipocytes.

Authors - Mutch D M DM

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kenzo (Verified Customer) (08-29-2023)

This antibody worked pretty well for IF on mouse liver frozen sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Liver