RNF156 Polyclonal antibody

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• RNF156 Polyclonal antibody (11285-1-AP)

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• KD/KO Validated

RNF156 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11285-1-AP Copied!

Publications (17)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

E3 ubiquitin-protein ligase MGRN1, EC:2.3.2.27, KIAA0544, mahogunin, ring finger 1, MGRN1

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  WB analysis using 11285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11285-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 11285-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11285-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11285-1-AP targets RNF156 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1808

  Product name: Recombinant human MGRN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC014199

  Sequence: SDDENSDNSNECVVCLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLEHDEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSRQKGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITEEVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPGRPTSMETAHGLATTSPTWPPLGGPSPDPSAAELTPL

• Full Name: mahogunin, ring finger 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 58-64 kDa
• GenBank Accession Number: BC014199
• Gene Symbol: MGRN1
• Gene ID (NCBI): 23295
• RRID: AB_2143351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60291
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MGRN1(E3 ubiquitin-protein ligase) mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. It also plays a role in the regulation of endosome-to-lysosome trafficking. Human and mouse mahogunin exhibit a similar expression pattern across multiple tissues with particularly high levels in brain(PMID: 12560552). It has 4 isoforms produced by alternative splicing with the molecular mass of 58-64 kDa.

Publications

What published studies show

The RNF156 Polyclonal antibody (Cat# 11285-1-AP) has 17 citations across journals including Mol Cell, Cell Death Dis, Cancer Lett, Dev Cell, Cell Mol Life Sci, J Biol Chem, Development, J Cell Sci, Pigment Cell Melanoma Res, Front Oncol, Mamm Genome, Int J Mol Sci, Biochim Biophys Acta, Cell Rep, and bioRxiv. Research fields span ubiquitin ligase biology, melanoma, ovarian cancer, Hedgehog signaling, neurodegeneration, obesity, mitochondrial homeostasis, cuproptosis, and melanocortin receptor regulation.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Cell; Design principles of a membrane-spanning ubiquitin ligase.; Authors - Carys Williams ; KO Validated
human	WB,IF	Mol Cell; Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival.; Authors - Stefano Benvegnù ; KD Validated
		Cell Death Dis; Endoplasmic reticulum stress related super-enhancers suppress cuproptosis via glycolysis reprogramming in lung adenocarcinoma; Authors - Yan Gu
human	WB	Cancer Lett; MGRN1 depletion promotes intercellular adhesion in melanoma by upregulation of E-cadherin and inhibition of CDC42; Authors - S Cerdido ; KD Validated
human	WB	Dev Cell; A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development.; Authors - Jennifer H Kong
		Cell Mol Life Sci; Aquaglyceroporin-7 ameliorates sorafenib resistance and immune evasion in hepatocellular carcinoma through inhibition of lipid accumulation; Authors - Guangsi He

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The product has received one review with a 5-star rating. The reviewer used it for Western Blot and reported that it works well, indicating satisfactory performance for this application.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Parijat (Verified Customer) (09-20-2023)

Works well.

• Applications: Western Blot