RNF156 Polyclonal antibody

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  WB analysis using 11285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11285-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11285-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11285-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

RNF156 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11285-1-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

KIAA0544, mahogunin, ring finger 1, MGRN1, RING finger protein 156, RNF156

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

11285-1-AP targets RNF156 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1808

  Product name: Recombinant human MGRN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC014199

  Sequence: SDDENSDNSNECVVCLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLEHDEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSRQKGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITEEVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPGRPTSMETAHGLATTSPTWPPLGGPSPDPSAAELTPL

• Full Name: mahogunin, ring finger 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 58-64 kDa
• GenBank Accession Number: BC014199
• Gene Symbol: MGRN1
• Gene ID (NCBI): 23295
• RRID: AB_2143351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60291
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MGRN1(E3 ubiquitin-protein ligase) mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. It also plays a role in the regulation of endosome-to-lysosome trafficking. Human and mouse mahogunin exhibit a similar expression pattern across multiple tissues with particularly high levels in brain(PMID: 12560552). It has 4 isoforms produced by alternative splicing with the molecular mass of 58-64 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Mol Cell; Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival.; Authors - Stefano Benvegnù ; KD Validated
human	WB	Dev Cell; A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development.; Authors - Jennifer H Kong
human	WB	Pigment Cell Melanoma Res; MGRN1-dependent pigment-type switching requires its ubiquitination activity but not its interaction with TSG101 or NEDD4.; Authors - Gunn Teresa M TM
human,mouse	IP	Development; Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength.; Authors - Jennifer H Kong
human	WB,IHC	Front Oncol; Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.; Authors - Xiao-Fei Li
mouse	WB,IF	J Biol Chem; Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein.; Authors - Overton John D JD ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (09-20-2023)

Works well.

• Applications: Western Blot