MGST1 Polyclonal antibody

MGST1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15906-1-AP

Synonyms

GST12, MGST, MGST I, MGST1, Microsomal GST 1, Microsomal GST I

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Validation Data Gallery

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  WB analysis using 15906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15906-1-AP (MGST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15906-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15906-1-AP (MGST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15906-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15906-1-AP (MGST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15906-1-AP (MGST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15906-1-AP (MGST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 15906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MGST1 antibody (15906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse lung tissue，rat liver tissue
• Positive IHC detected in: human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15906-1-AP targets MGST1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGST1 fusion protein Ag8498
• Full Name: microsomal glutathione S-transferase 1
• Calculated Molecular Weight: 155 aa, 18 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC005923
• Gene Symbol: MGST1
• Gene ID (NCBI): 4257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microsomal glutathione S-transferase 1 (MGST1) is a membrane-bound transferase involved in exogenous detoxification and protection of cells from oxidative stress. MGST1 is mainly localized in the mitochondria, the plasma membrane of the endoplasmic reticulum (ER). The active form of MGST1 is a noncovalently associated homotrimer that binds one substrate glutathione molecule/trimer (PMID: 14726533; PMID: 11101503). The theoretical molecular weight of MGST1 is 17 kDa, and its homomeric molecular weight is 51-53 kDa.