Tested Applications
| Positive IHC detected in | human skin cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15734-1-AP targets MIA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8499 Product name: Recombinant human MIA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-131 aa of BC005910 Sequence: GGPMPKLADRKLCADQECSHPISMAVALQDYMAPDCRFLTIHRGQVVYVFSKLKGRGRLFWGGSVQGDYYGDLAARLGYFPSSIVREDQTLKPGKVDVKTDKWDFYCQ Predict reactive species |
| Full Name | melanoma inhibitory activity |
| Calculated Molecular Weight | 131 aa, 15 kDa |
| GenBank Accession Number | BC005910 |
| Gene Symbol | MIA |
| Gene ID (NCBI) | 8190 |
| RRID | AB_2878176 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Melanoma-derived growth regulatory protein, also known as MIA, CD RAP, is a protein that in humans is encoded by the MIA gene. It is a marker for malignant melanoma (PMID: 9242442).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MIA antibody 15734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MIA Polyclonal antibody (Cat# 15734-1-AP) has a total of 2 citations. These appear in Developmental Cell (2025) and Journal of Clinical Medicine (2021). The cited research covers oncogenesis and ER-phagy in pancreatic epithelial cells, as well as MIA as an immunohistochemical marker for oral epithelial dysplasia. Applications used include Western blotting and IHC, with species spanning mouse and human.
| Species | Application | Title |
|---|---|---|
Dev Cell ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis. | ||
J Clin Med Melanoma Inhibitory Activity and Melanoma Inhibitory Activity 2 as Novel Immunohistochemical Markers of Oral Epithelial Dysplasia. |
