MIA3 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86403-3-RR (same clone as 86403-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86403-3-RR (MIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86403-3-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 86403-3-RR (same clone as 86403-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIA3 antibody (86403-3-RR, Clone: 250698A6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86403-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86403-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86403-3-RR against Human MIA3 were performed. The affinity constant is below 1 pM.

MIA3 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86403-3-PBS

Clone No.250698A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ARNT, C219 reactive peptide, C219-reactive peptide, D320, KIAA0268

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Product Information

86403-3-PBS targets MIA3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag39740

  Product name: Recombinant human MIA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 118-467 aa of BC047116

  Sequence: KAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRCPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGEPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

• Full Name: melanoma inhibitory activity family, member 3
• Calculated Molecular Weight: 214 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: BC047116
• Gene Symbol: MIA3
• Gene ID (NCBI): 375056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5JRA6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.