MICALL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MICALL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 24408-1-AP

Synonyms

JRAB, MICAL L2, MICAL like 2, MICAL like protein 2, MICALL2

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Validation Data Gallery

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  WB analysis of PC-3 using 24408-1-AP

  WB result of MICALL2 antibody (24408-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MICALL2 transfected PC-3 cells.

• 

  WB analysis using 24408-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24408-1-AP (MICALL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24408-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24408-1-AP (MICALL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: DU 145 cells, PC-3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

24408-1-AP targets MICALL2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MICALL2 fusion protein Ag19931
• Full Name: MICAL-like 2
• Calculated Molecular Weight: 904 aa, 98 kDa
• Observed Molecular Weight: 109 kDa
• GenBank Accession Number: BC037988
• Gene Symbol: MICALL2
• Gene ID (NCBI): 79778
• RRID: AB_2879527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Int J Mol Sci; Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion; Authors - Tianxiang Xia ; KD Validated
human	WB,IHC,IF	Biochem Pharmacol; MICAL-L2, as an estrogen-responsive gene, is involved in ER-positive breast cancer cell progression and tamoxifen sensitivity via the AKT/mTOR pathway; Authors - Pushuai Wen ; KD Validated
human	WB,IHC,IF,IP	Cell Commun Signal; MICALL2 as a substrate of ubiquitinase TRIM21 regulates tumorigenesis of colorectal cancer; Authors - Pushuai Wen ; KD Validated
mouse	IF	Arch Biochem Biophys; MICALL2 participates in the regulation of epithelial-mesenchymal transition in alveolar epithelial cells - Potential roles in pulmonary fibrosis; Authors - Yingying Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

WB required long exposure >100s. Multiple background bands. Molecular weight is BioRad Precision Plus.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

MICALL2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:24408-1-AP)