MICB Polyclonal antibody

MICB Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 14325-1-AP

Synonyms

PERB11.2, MIC-B, MIC B, MHC class I polypeptide-related sequence B

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Validation Data Gallery

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  IHC staining of human colon cancer using 14325-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14325-1-AP (MICB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14325-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14325-1-AP (MICB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 14325-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14325-1-AP (MICB Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14325-1-AP targets MICB in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MICB fusion protein Ag5591
• Full Name: MHC class I polypeptide-related sequence B
• Calculated Molecular Weight: 43 kDa
• GenBank Accession Number: BC044218
• Gene Symbol: MICB
• Gene ID (NCBI): 4277
• RRID: AB_2878045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.