Tested Applications
| Positive WB detected in | PC-12 cells, BxPC-3 cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18939-1-AP targets MIDN in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13492 Product name: Recombinant human MIDN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 127-468 aa of BC094778 Sequence: TQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAAAAARGDPSIASPVSSPCRPVSSAARVPPVPTSPSPASPSPITAGSFRSHAASTTCPEQMDCSPTASSSASPGASTTSTPGASPAPRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLAQLRCHAQCSPASPAPDLAPRTTSCEKLTAAPSASLLQGQSQIRMCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWSPSRKAGRSDSSSSGGGGSPSEASGLGLDFEDSVWKPEANPDIKSEFVVA Predict reactive species |
| Full Name | midnolin |
| Calculated Molecular Weight | 468 aa, 49 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC094778 |
| Gene Symbol | MIDN |
| Gene ID (NCBI) | 90007 |
| RRID | AB_2878569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q504T8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Midbrain nucleolar protein (midnolin, MIDN) is abundantly expressed in embryonic midbrain and localizes in the nucleus and nucleolus. MIDN mRNA was strongly and abundantly expressed during early developmental stages, and that the expression was not limited to midbrain but also expressed in diverse adult mouse tissues, including heart, spleen, lung, liver, skeletal muscle, kidney and testis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIDN antibody 18939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Midnolin antibody (Cat# 18939-1-AP) has 9 citations across journals including Science, PNAS, EMBO Reports, Cancer Science, J Cell Mol Med, Biomedicines, Acta Biochim Biophys Sin, J Virol, and Mol Cell Biol. Research fields span ubiquitination-independent protein degradation via the midnolin-proteasome pathway, c-Myc degradation, cancer progression (prostate and liver), myoblast differentiation, coronavirus inhibition, and beta cell gene regulation. Applications include WB, CoIP, IHC, IF, and IP in human, mouse, and pig samples.
| Species | Application | Title |
|---|---|---|
Science The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation
| ||
Proc Natl Acad Sci U S A Targeted degradation of c-Myc through the midnolin-proteasome pathway.
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EMBO Rep Matrix stiffness induces midnolin-dependent lamin B1 degradation to control myoblast differentiation.
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Cancer Sci Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling | ||
J Cell Mol Med Midnolin Correlates With Anti-Tumour Immunity and Promotes Liver Cancer Progression Through β-Catenin | ||
Biomedicines Comprehensive Analysis Reveals Midnolin as a Potential Prognostic, Therapeutic, and Immunological Cancer Biomarker
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