MIER2 Polyclonal antibody

MIER2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17426-1-AP

Synonyms

Mi-er2, Mesoderm induction early response protein 2, KIAA1193

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Validation Data Gallery

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  WB analysis using 17426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17426-1-AP (MIER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17426-1-AP (MIER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17426-1-AP targets MIER2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIER2 fusion protein Ag11491
• Full Name: mesoderm induction early response 1, family member 2
• Calculated Molecular Weight: 545 aa, 60 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC028203
• Gene Symbol: MIER2
• Gene ID (NCBI): 54531
• RRID: AB_2143931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).