MIER3 Polyclonal antibody

MIER3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17543-1-AP

Synonyms

Mi er3, MIER3

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Validation Data Gallery

• 

  WB analysis of mouse uterus using 17543-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17543-1-AP (MIER3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 17543-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17543-1-AP (MIER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse uterus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17543-1-AP targets MIER3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIER3 fusion protein Ag11604
• Full Name: mesoderm induction early response 1, family member 3
• Calculated Molecular Weight: 522 aa, 59 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC041348
• Gene Symbol: MIER3
• Gene ID (NCBI): 166968
• RRID: AB_2143938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mesoderm induction early response (MIER) protein family is known as fibroblast growth factor (FGF)-regulated immediate-early protein family. Activated by FGF, MIER proteins may have a pivotal role in FGF-regulated cellular activities, indicating a potential influence in the progression of certain cancers [PMID:15117948]. MIER3 localizes to the nucleus and primarily functions as a transcriptional repressor. Rat mammary carcinoma susceptibility 1b (Mcs1b) is a quantitative trait locus on RN02 that confers decreased susceptibility when Copenhagen (COP)-resistant alleles are introgressed into a Wistar Furth (WF)-susceptible genome. MIER3 is a candidate Mcs1b gene [PMID:22993404].

Publications

Species	Application	Title
mouse	WB	Mitochondrion; Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5.; Authors - Shih-Wei Chen