Tested Applications
| Positive WB detected in | mouse uterus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17543-1-AP targets MIER3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11604 Product name: Recombinant human MIER3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 177-522 aa of BC041348 Sequence: DQLLWCPDVVLESKVKEYLVETSLRTGSEKIMDRISAGTHTRDNEQALYELLKCNHNIKEAIERYCCNGKASQGMTAWTEEECRSFEHALMLFGKDFHLIQKNKVRTRTVAECVAFYYMWKKSERYDYFAQQTRFGKKRYNHHPGVTDYMDRLVDETEALGGTVNASALTSNRPEPIPDQQLNILNSFTASDLTALTNSVATVCDPTDVNCLDDSFPPLGNTPRGQVNHVPVVTEELLTLPSNGESDCFNLFETGFYHSELNPMNMCSEESERPAKRLKMGIAVPESFMNEVSVNNLGVDFENHTHHITSAKMAVSVADFGSLSANETNGFISAHALHQHAALHSE Predict reactive species |
| Full Name | mesoderm induction early response 1, family member 3 |
| Calculated Molecular Weight | 522 aa, 59 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC041348 |
| Gene Symbol | MIER3 |
| Gene ID (NCBI) | 166968 |
| RRID | AB_2143938 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z3K6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The mesoderm induction early response (MIER) protein family is known as fibroblast growth factor (FGF)-regulated immediate-early protein family. Activated by FGF, MIER proteins may have a pivotal role in FGF-regulated cellular activities, indicating a potential influence in the progression of certain cancers [PMID:15117948]. MIER3 localizes to the nucleus and primarily functions as a transcriptional repressor. Rat mammary carcinoma susceptibility 1b (Mcs1b) is a quantitative trait locus on RN02 that confers decreased susceptibility when Copenhagen (COP)-resistant alleles are introgressed into a Wistar Furth (WF)-susceptible genome. MIER3 is a candidate Mcs1b gene [PMID:22993404].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIER3 antibody 17543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MIER3 Polyclonal antibody (Cat# 17543-1-AP) has 2 citations. They appear in Mitochondrion (2013) and Scientific Reports (2026). The research fields include mitochondrial protein maturation (COQ5) and the MIER corepressor complex in embryonic development and cell migration. Both studies used the antibody for Western blot (WB) applications, with the latter spanning mouse, human, and zebrafish species.
| Species | Application | Title |
|---|---|---|
Mitochondrion Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5. | ||
Sci Rep The C16orf87 protein is a subunit of the MIER corepressor complex controlling embryonic development and cell migration |
