Product Information
24288-1-PBS targets MIF4GD in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17931 Product name: Recombinant human MIF4GD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-256 aa of BC033759 Sequence: EPSREEYKIQSFDAETQQLLKTALKVACFETEDGEYSVCQRSYSNCSRLMPSRCNTQYRDPGAVDLEKVANVIVDHSLQDCVFSKEAGRMCYAIIQAESKQAGQSVFRRGLLNRLQQEYQAREQLRARSLQGWVCYVTFICNIFDYLRVNNMPMMALVNPVYDCLFRLAQPDSLSKEEEVDCLVLQLHRVGEQLEKMNGQRMDELFVLIRDGFLLPTGLSSLAQLLLLEIIEFRAAGWKTTPAAHKYYYSEVSD Predict reactive species |
| Full Name | MIF4G domain containing |
| Calculated Molecular Weight | 256 aa, 29 kDa |
| Observed Molecular Weight | ~25 kDa |
| GenBank Accession Number | BC033759 |
| Gene Symbol | MIF4GD |
| Gene ID (NCBI) | 57409 |
| RRID | AB_3742106 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A9UHW6 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
MIF4GD (MIF4G domain containing), also known as MIFD, AD023, or SLIP1, is a 222 amino acid protein that contains one MIF4G domain. Localized to both the nucleus and the cytoplasm, MIF4GD plays a role in the replication-dependent translation of histone mRNAs, which differ from most eukaryotic mRNAs in that they end with a stem-loop instead of a poly-A tail. Specifically, MIF4GD interacts with SLBP, eIF4G and DAP-5. Via its interaction with SLBP, MIF4GD is thought to be tethered to the stem loops of histone mRNAs where it may facilitate the circularizing of the mRNAs, thereby enhancing their translation. Depletion of MIF4GD results in reduced histone translation and may lead to cell death, suggesting that MIF4GD plays an important role in cell survival. Two isoforms of MIF4GD exist due to alternative splicing events.
