MIG6; ERRFI1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83475-2-RR (same clone as 83475-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83475-2-RR (MIG6; ERRFI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83475-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83475-2-RR (same clone as 83475-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MIG6; ERRFI1 Recombinant antibody (83475-2-RR, Clone:240447C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83475-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83475-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83475-2-RR against Human MIG6; ERRFI1 were performed. The affinity constant is 2.04 nM.

MIG6; ERRFI1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 83475-2-PBS

Clone No.240447C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ERRFI1, 240447C3, GENE 33, MIG 6, MIG6

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Product Information

83475-2-PBS targets MIG6; ERRFI1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33341

  Product name: Recombinant human MIG6; ERRFI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 113-462 aa of NM_018948

  Sequence: CGFKKLTVNGVCASTPPLTPIKNSPSLFPCAPLCERGSRPLPPLPISEALSLDDTDCEVEFLTSSDTDFLLEDSTLSDFKYDVPGRRSFRGCGQINYAYFDTPAVSAADLSYVSDQNGGVPDPNPPPPQTHRRLRRSHSGPAGSFNKPAIRISNCCIHRASPNSDEDKPEVPPRVPIPPRPVKPDYRRWSAEVTSSTYSDEDRPPKVPPREPLSPSNSRTPSPKSLPSYLNGVMPPTQSFAPDPKYVSSKALQRQNSEGSASKVPCILPIIENGKKVSSTHYYLLPERPPYLDKYEKFFREAEETNGGAQIQPLPADCGISSATEKPDSKTKMDLGGHVKRKHLSYVVSP*

• Full Name: ERBB receptor feedback inhibitor 1
• Calculated Molecular Weight: 51kd
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: NM_018948
• Gene Symbol: MIG6
• Gene ID (NCBI): 54206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UJM3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.