Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lymphoma tissue, human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13137-1-AP targets MINK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3795 Product name: Recombinant human MINK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-304 aa of BC034673 Sequence: MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKD Predict reactive species |
| Full Name | misshapen-like kinase 1 (zebrafish) |
| Calculated Molecular Weight | 1332 aa, 150 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC034673 |
| Gene Symbol | MINK1 |
| Gene ID (NCBI) | 50488 |
| RRID | AB_10644151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N4C8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Misshapen/Nck-interacting kinase (NIK)-related kinase 1 (MINK1), also known as mitogen-activated protein 4 kinase 6, a serine-threonine protein kinase belonging to the sterile 20 kinase superfamily, has been implicated in diverse physiological and pathophysiological processes, including platelet activation and thrombus formation (PMID: 38723371). MINK1 has the potential to modulate the activation of NLRP3 inflammasome, indicating its pivotal role in governing pyroptosis. Overall, MINK1 is known to regulate cell senescence, cell motility and migration (PMID: 36182968). MINK1 expression has been demonstrated in multiple tissues and organs, including nervous tissue, kidneys, cancer tissues, and immune system cells. (PMID: 39727954)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MINK1 antibody 13137-1-AP | Download protocol |
| IP protocol for MINK1 antibody 13137-1-AP | Download protocol |
| WB protocol for MINK1 antibody 13137-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Lung adenocarcinoma cell-derived exosomes promote M2 macrophage polarization through transmission of miR-3153 to activate the JNK signaling pathway | ||
Cardiovasc Ther Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats. | ||
Front Genet MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma | ||
Front Immunol Resveratrol contributes to NK cell-mediated breast cancer cytotoxicity by upregulating ULBP2 through miR-17-5p downmodulation and activation of MINK1/JNK/c-Jun signaling |
