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WB analysis using 13137-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13137-1-AP (MINK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13137-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13137-1-AP (MINK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 13137-1-AP
IP result of anti-MINK1 (IP:13137-1-AP, 4ug; Detection:13137-1-AP 1:300) with mouse brain tissue lysate 4000ug.
IHC staining of human lymphoma using 13137-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lymphoma using 13137-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 13137-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 13137-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 13137-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse brain using 13137-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

MINK1 Polyclonal antibody

Name: MINK1 Polyclonal antibody
Brand: Proteintech
SKU: 13137-1-AP

MINK1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13137-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

B55, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6, MEK kinase kinase 6, MEKKK 6, MINK, MINK1, Misshapen like kinase 1, Misshapen/NIK related kinase, YSK2, ZC3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lymphoma tissue, human skeletal muscle tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

13137-1-AP targets MINK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3795 Product name: Recombinant human MINK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-304 aa of BC034673 Sequence: MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKD Predict reactive species
Full Name	misshapen-like kinase 1 (zebrafish)
Calculated Molecular Weight	1332 aa, 150 kDa
Observed Molecular Weight	150 kDa
GenBank Accession Number	BC034673
Gene Symbol	MINK1
Gene ID (NCBI)	50488
RRID	AB_10644151
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N4C8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MINK1 antibody 13137-1-AP	Download protocol
IP protocol for MINK1 antibody 13137-1-AP	Download protocol
WB protocol for MINK1 antibody 13137-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Hum Mol Genet Lung adenocarcinoma cell-derived exosomes promote M2 macrophage polarization through transmission of miR-3153 to activate the JNK signaling pathway Authors - Lu Xu View Article
rat	WB	Cardiovasc Ther Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats. Authors - Xuemei Luo View Article
mouse	IHC	Front Genet MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma Authors - Zhifeng Chen View Article
human	WB	Front Immunol Resveratrol contributes to NK cell-mediated breast cancer cytotoxicity by upregulating ULBP2 through miR-17-5p downmodulation and activation of MINK1/JNK/c-Jun signaling Authors - Bisha Ding View Article