MIPOL1 Polyclonal antibody

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  WB analysis of HepG2 using 14158-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14158-1-AP (MIPOL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14158-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14158-1-AP (MIPOL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14158-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14158-1-AP (MIPOL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14158-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14158-1-AP (MIPOL1 antibody) at dilution of 1:100 (under 40x lens).

MIPOL1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14158-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MIPOL1, mirror image polydactyly 1

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14158-1-AP targets MIPOL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5340

  Product name: Recombinant human MIPOL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-261 aa of BC035870

  Sequence: MSRLQLAIEERDEAIARAKHMEMSLKVLENINPEENDMTLQELLNRINNADTGIAIQKNGAIIVDRIYKTKECKMRITAEEMSALIEERDAALSKCKRLEQELHHVKEQNQTSANNMRHLTAENNQERALKAKLLSMQQARETAVQQYKKLEEEIQTLRVYYSLHKSLSQEENLKDQFNYTLSTYEEALKNRENIVSITQQQNEELATQLQQALTERANMELQLQHAREASQVANEKVQKLERLVDVLRKKVGTGTMRTVI

• Full Name: mirror-image polydactyly 1
• Calculated Molecular Weight: 442 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC035870
• Gene Symbol: MIPOL1
• Gene ID (NCBI): 145282
• RRID: AB_2141930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TD10
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.