MKK7 Polyclonal antibody

• Featured Product
• KD/KO Validated

MKK7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 55030-1-AP

Synonyms

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, PRKMK7

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 55030-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55030-1-AP (MKK7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 55030-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55030-1-AP (MKK7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55030-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55030-1-AP (MKK7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 55030-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 55030-1-AP (MKK7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

55030-1-AP targets MKK7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 7
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_145185
• Gene Symbol: MAP2K7
• Gene ID (NCBI): 5609
• RRID: AB_2881257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP, WB	Front Physiol; Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.; Authors - Changjiang Ying
human	WB	PLoS One; A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs.; Authors - Zhuoying Li
mouse	WB,IF	Gene; ANXA1 affects murine hair follicle growth through EGF signaling pathway.; Authors - Hao Zhang
human	WB	Curr Med Sci; Raf/MEK/ERK Signaling Pathway Is Involved in the Inhibition of Glioma Cell Proliferation and Invasion in the Ketogenic Microe-nvironment; Authors - Wen-Tao Fan
human	WB,IHC	Onco Targets Ther; miR-125b inhibited epithelial-mesenchymal transition of triple-negative breast cancer by targeting MAP2K7.; Authors - Liquan Hong ; KD Validated
human	WB	BMC Cancer; MicroRNA-148b regulates tumor growth of non-small cell lung cancer through targeting MAPK/JNK pathway.; Authors - Lin Lu