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  • KD/KO Validated

MKK7 Polyclonal antibody

MKK7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55030-1-AP

Synonyms

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, PRKMK7



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55030-1-AP targets MKK7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase 7
Calculated molecular weight 52 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_145185
Gene symbol MAP2K7
Gene ID (NCBI) 5609
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MKK7 antibody 55030-1-APDownload protocol
IHC protocol for MKK7 antibody 55030-1-APDownload protocol
IF protocol for MKK7 antibody 55030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cancer

MicroRNA-148b regulates tumor growth of non-small cell lung cancer through targeting MAPK/JNK pathway.

Authors - Lin Lu
humanWB,IHC

Onco Targets Ther

miR-125b inhibited epithelial-mesenchymal transition of triple-negative breast cancer by targeting MAP2K7.

Authors - Liquan Hong
  • KD Validated
humanWB

PLoS One

A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs.

Authors - Zhuoying Li