Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, Y79 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14735-1-AP targets Muskelin in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6147 Product name: Recombinant human MKLN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 388-735 aa of BC067825 Sequence: FDHQMCMDSEKHMIYTFGGRILTCNGSVDDSRASEPQFSGLFAFNCQCQTWKLLREDSCNAGPEDIQSRIGHCMLFHSKNRCLYVFGGQRSKTYLNDFFSYDVDSDHVDIISDGTKKDSGMVPMTGFTQRATIDPELNEIHVLSGLSKDKEKREENVRNSFWIYDIVRNSWSCVYKNDQAAKDNPTKSLQEEEPCPRFAHQLVYDELHKVHYLFGGNPGKSCSPKMRLDDFWSLKLCRPSKDYLLRHCKYLIRKHRFEEKAQVDPLSALKYLQNDLYITVDHSDPEETKEFQLLASALFKSGSDFTALGFSDVDHTYAQRTQLFDTLVNFFPDSMTPPKGNLVDLITL Predict reactive species |
| Full Name | muskelin 1, intracellular mediator containing kelch motifs |
| Calculated Molecular Weight | 735 aa, 85 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC067825 |
| Gene Symbol | Muskelin |
| Gene ID (NCBI) | 4289 |
| RRID | AB_2142504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL63 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Muskelin antibody 14735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Muskelin Polyclonal antibody (Cat# 14735-1-AP) has 4 citations published in Neuron, Molecular Cell, Cell Reports, and Scientific Reports. Research fields include prion disease progression (PrPC lysosome/exosome targeting), mTORC1-CTLH E3 ligase-mediated protein degradation (HMG-CoA synthase 1, UCK2 turnover), and Ran Binding Protein 9–Nucleolin interaction. Applications cited: WB, IP, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Neuron Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.
| ||
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway
| ||
Cell Rep mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase | ||
Sci Rep Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. |
Reviews
What customers say
Both reviewers used the antibody for Western Blot at a 1:1000 dilution and confirmed target band detection in HEK293T cells (one also tested HCT116). However, both noted that the signal intensity was relatively weak. One reviewer suggested the low signal might be attributable to low protein expression levels and recommended KO validation for stronger confirmation. Overall, the antibody performs adequately but delivers modest signal strength in standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (12-31-2021) | This antibody is ok, but not the best. At least, KO validation. The weak signal could be due to the low protein expression level though.
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H (Verified Customer) (04-05-2020) | Primary antibody: 1:1000 dilution in 5% BSA, over night at 4CSecondary antibody: Rabbit IRDye800Detection: odyssey ClxI was able to detect the band in HEK293T and HCT116 cells. However, the band intensity was not that strong.
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