MKNK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MKNK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17354-1-AP

Synonyms

MNK2, MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7

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Validation Data Gallery

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  WB analysis of mouse spleen using 17354-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17354-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17354-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17354-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 17354-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (17354-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

17354-1-AP targets MKNK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MKNK2 fusion protein Ag10546
• Full Name: MAP kinase interacting serine/threonine kinase 2
• Calculated Molecular Weight: 465 aa, 52 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC018345
• Gene Symbol: MKNK2
• Gene ID (NCBI): 2872
• RRID: AB_2878395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.

Publications

Species	Application	Title
human		Clin Sci (Lond); Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.; Authors - Zhiling Luo
human	WB	Oncotarget; Impact of eIF4E phosphorylation at Ser209 via MNK2a on tumour recurrence after curative surgery in localized clear cell renal cell carcinoma.; Authors - Osamu Ichiyanagi
human	WB	Oncotarget; Inhibitory effects of Tomivosertib in acute myeloid leukemia.; Authors - Milagros Suarez
human,mouse	WB,IHC	Inflammation; High Expression of miR-6785-5p in the Serum Exosomes of Psoriasis Patients Alleviates Psoriasis-Like Skin Damage by Interfering with the MNK2/p-eIF4E Axis in Keratinocytes; Authors - Ruijie Wang ; KD Validated