MKP-2 Monoclonal antibody

MKP-2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G1C12

Cat no : 66349-1-Ig

Synonyms

dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2



Tested Applications

Positive WB detected inpig brain tissue, HepG2 cells, PC-12 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66349-1-Ig targets MKP-2 in WB, IHC, IF,ELISA applications and shows reactivity with human, pig, rat, mouse samples.

Tested Reactivity human, pig, rat, mouse
Cited Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MKP-2 fusion protein Ag1206
Full Name dual specificity phosphatase 4
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa, 33 kDa
GenBank accession numberBC014565
Gene symbol DUSP4
Gene ID (NCBI) 1846
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).

Protocols

Product Specific Protocols
WB protocol for MKP-2 antibody 66349-1-IgDownload protocol
IHC protocol for MKP-2 antibody 66349-1-IgDownload protocol
IF protocol for MKP-2 antibody 66349-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cancer

PMID: 31258782

Authors - Xianqing Chen
ratIHC

Mol Neurobiol

Dual Specificity Phosphatases Support Axon Plasticity and Viability.

Authors - Ambika Chandrasekhar