CoraLite®594-conjugated MKP-2 Monoclonal antibody

MKP-2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G1C12

Cat no : CL594-66349

Synonyms

dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66349

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66349 (MKP-2 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66349 targets MKP-2 in IF applications and shows reactivity with human, pig, rat, mouse samples.

• Tested Reactivity: human, pig, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MKP-2 fusion protein Ag1206
• Full Name: dual specificity phosphatase 4
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa, 33 kDa
• GenBank Accession Number: BC014565
• Gene Symbol: DUSP4
• Gene ID (NCBI): 1846
• RRID: AB_2883565
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).