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  • KD/KO Validated

MKRN2 Polyclonal antibody

MKRN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12238-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

RING-type E3 ubiquitin transferase makorin-2, RING finger protein 62, makorin ring finger protein 2, HSPC070, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, A375 cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12238-1-AP targets MKRN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MKRN2 fusion protein Ag2881 Predict reactive species
Full Name makorin ring finger protein 2
Calculated Molecular Weight 416 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC015715
Gene Symbol MKRN2
Gene ID (NCBI) 23609
RRIDAB_2142636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H000
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MKRN2(Probable E3 ubiquitin-protein ligase makorin-2) is also named as RNF62. It is an E3 ubiquitin ligase that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It may be playing a functional role in negatively regulating neurogenesis via PI3K/Akt signaling.

Protocols

Product Specific Protocols
WB protocol for MKRN2 antibody 12238-1-APDownload protocol
IHC protocol for MKRN2 antibody 12238-1-APDownload protocol
IF protocol for MKRN2 antibody 12238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Immunol

The RNA-binding E3 ligase MKRN2 selectively disrupts Il6 translation to restrain inflammation

Authors - Zhou Yu
humanWB

Biochem Biophys Res Commun

Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.

Authors - Caiwei Jia
  • KD Validated
humanWB

Oncol Lett

Ubiquitination of P53 by E3 ligase MKRN2 promotes melanoma cell proliferation.

Authors - Yiling Zhang
  • KD Validated
humanWB

J Invest Dermatol

N6-methyladenosine modification of lnc-CCKAR-5 regulates autophagy in human umbilical cord mesenchymal stem cells by destabilizing LMNA and inhibits diabetic wound healing

Authors - Jian Wang
humanWB

Cancer Biomark

MKRN1/2 serve as tumor suppressors in renal clear cell carcinoma by regulating the expression of p53

Authors - Yun Yang
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