MKX Polyclonal antibody

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  WB analysis using 27012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27012-1-AP (MKX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MKX Polyclonal Antibody for WB, ELISA

Cat No. 27012-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C10orf48, Homeobox protein Mohawk, IFRX, IRXL1, mohawk homeobox

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27012-1-AP targets MKX in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MKX fusion protein Ag25549
• Full Name: mohawk homeobox
• Calculated Molecular Weight: 352 aa, 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC036207
• Gene Symbol: MKX
• Gene ID (NCBI): 283078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IYA7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mohawk (Mkx) is a member of the Three Amino Acid Loop Extension (TALE) superclass of atypical homeobox genes. It encodes a transcriptional repressor that is expressed in the embryonic precursors of skeletal muscle, tendon, cartilage, and bone. This gene is highly conserved across species and is known to be involved in the differentiation and maintenance of these tissues.