MLF1 Polyclonal antibody

MLF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13100-1-AP

Synonyms

MLF1, myeloid leukemia factor 1

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Validation Data Gallery

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  WB analysis of mouse heart using 13100-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13100-1-AP (MLF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13100-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13100-1-AP (MLF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13100-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13100-1-AP (MLF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 13100-1-AP

  IP result of anti-MLF1 (IP:13100-1-AP, 4ug; Detection:13100-1-AP 1:500) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 13100-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13100-1-AP (MLF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13100-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13100-1-AP (MLF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human brain tissue, mouse testis tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13100-1-AP targets MLF1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLF1 fusion protein Ag3727
• Full Name: myeloid leukemia factor 1
• Calculated Molecular Weight: 268 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC007045
• Gene Symbol: MLF1
• Gene ID (NCBI): 4291
• RRID: AB_2147670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs).; Authors - Daoyuan Huang