Tested Applications
| Positive WB detected in | Caco-2 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 17 publications below |
| IHC | See 13 publications below |
| IF | See 8 publications below |
| CoIP | See 1 publications below |
Product Information
11697-1-AP targets MLH1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2319 Product name: Recombinant human MLH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 407-756 aa of BC006850 Sequence: QPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC Predict reactive species |
| Full Name | mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) |
| Calculated Molecular Weight | 756 aa, 85 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC006850 |
| Gene Symbol | MLH1 |
| Gene ID (NCBI) | 4292 |
| RRID | AB_2145604 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40692 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MLH1 antibody 11697-1-AP | Download protocol |
| IP protocol for MLH1 antibody 11697-1-AP | Download protocol |
| WB protocol for MLH1 antibody 11697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer | ||
Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. | ||
Nucleic Acids Res Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing. | ||
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. | ||
Nucleic Acids Res Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch. | ||
Cancer Lett Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Elena (Verified Customer) (06-26-2025) | Works for us for IP
|
FH Manohar (Verified Customer) (12-18-2021) | No non-specific binding, blocked with milk for 1 hour and antibody incubation at 4degrees over night
|
