Tested Applications
| Positive WB detected in | Caco-2 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11697-1-AP targets MLH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2319 Product name: Recombinant human MLH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 407-756 aa of BC006850 Sequence: QPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC Predict reactive species |
| Full Name | mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) |
| Calculated Molecular Weight | 756 aa, 85 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC006850 |
| Gene Symbol | MLH1 |
| Gene ID (NCBI) | 4292 |
| RRID | AB_2145604 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40692 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MLH1 antibody 11697-1-AP | Download protocol |
| IHC protocol for MLH1 antibody 11697-1-AP | Download protocol |
| IP protocol for MLH1 antibody 11697-1-AP | Download protocol |
| WB protocol for MLH1 antibody 11697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MLH1 Polyclonal antibody (Cat# 11697-1-AP) has 43 citations spanning journals including Nature Communications, Cell Metabolism, PNAS, Nucleic Acids Research, Cell Reports, and Advanced Science. Research fields covered include DNA mismatch repair, Lynch syndrome, and various cancers (colorectal, gastric, bladder, breast, endometrial, ovarian, glioma), as well as meiosis, spermatogenesis, and Huntington's disease. Cited applications include WB, IHC, IF, and CoIP.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. | ||
Cell Metab Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer | ||
Drug Resist Updat Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane | ||
Nat Commun Aberrant cytoplasmic localization of MLH1 characterizes a cell population that seeds breast cancer recurrence.
| ||
Nucleic Acids Res Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing. |
Reviews
What customers say
Both reviewers rated the product 4 out of 5 stars. One user confirmed it works well for immunoprecipitation. Another reported successful Western Blot results on HEK293 cells at a 1:1000 dilution, noting clean bands with no non-specific binding when using a 1-hour milk block and overnight 4°C antibody incubation. Overall, users find the antibody reliable and specific for its intended applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Elena (Verified Customer) (06-26-2025) | Works for us for IP
|
Manohar (Verified Customer) (12-18-2021) | No non-specific binding, blocked with milk for 1 hour and antibody incubation at 4degrees over night
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