MLH3 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• MLH3 Polyclonal antibody (25298-1-AP)

• Featured Product
• KD/KO Validated

MLH3 Polyclonal Antibody for WB, ELISA

Cat No. 25298-1-AP Copied!

Publications (3)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

HNPCC7, MLH3, mutL homolog 3 (E. coli), MutL protein homolog 3

• 

  WB analysis of HepG2 using 25298-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25298-1-AP (MLH3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 25298-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25298-1-AP (MLH3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for MLH3 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25298-1-AP targets MLH3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18201

  Product name: Recombinant human MLH3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1190-1430 aa of BC112167

  Sequence: QVLQQVDNKFIACLMSTKTEENGEADSYEKQQAQGSGRKKLLSSTLIPPLEITVTEEQRRLLWCYHKNLEDLGLEFVFPDTSDSLVLVGKVPLCFVEREANELRRGRSTVTKSIVEEFIREQLELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDGLSLQESCRLIEALSSCQLPFQCAHGRPSMLPLADIDHLEQEKQIKPNLTKLRKMAQAWRLFGKAECDTRQSLQQSMPPCEPP

• Full Name: mutL homolog 3 (E. coli)
• Calculated Molecular Weight: 1453 aa, 164 kDa
• Observed Molecular Weight: 164 kDa
• GenBank Accession Number: BC112167
• Gene Symbol: MLH3
• Gene ID (NCBI): 27030
• RRID: AB_2880015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHC1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The MLH3 Polyclonal antibody (Cat# 25298-1-AP) has 3 total citations. These appear in Progress in Neurobiology, Gastroenterology Reports, and Human Molecular Genetics. The cited research spans neurodegeneration (Huntington's disease CAG repeat expansion in mouse models) and human genetics/oncology (germline MLH3 splicing mutation associated with infertility, colon polyposis, and synchronous colon cancers). Applications used include Western blotting and immunohistochemistry.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Prog Neurobiol; Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice; Authors - Dazhang Bai
		Gastroenterol Rep (Oxf); A case of homozygous germline mutation at the MLH3 splicing site causing infertility, colon polyposis with synchronous multiple colon cancers, and osteoma.; Authors - Jiabo Gu
human	WB, IHC	Hum Mol Genet; Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.; Authors - Dazhang Bai ; KD Validated