Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29278-1-AP targets KMT2A/MLL1 in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30468 Product name: Recombinant human MLL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3520-3670 aa of NM_001197104 Sequence: SSGQRSASPSVPGPTKPKPKTKRFQLPLDKGNGKKHKVSHLRTSSSEAHIPDQETTSLTSGTGTPGAEAEQQDTASVEQSSQKECGQPAGQVAVLPEVQVTQNPANEQESAEPKTVEEEESNFSSPLMLWLQQEQKRKESITEKKPKKGLV Predict reactive species |
| Full Name | myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) |
| Calculated Molecular Weight | 432 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | NM_001197104 |
| Gene Symbol | MLL |
| Gene ID (NCBI) | 4297 |
| RRID | AB_2918266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q03164 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KMT2A, also known as mixed-lineage leukemia (MLL), is a gene that encodes a lysine methyltransferase responsible for the transcriptional activation through lysine 4 of histone 3 (H3K4) methylation. KMT2A is expressed mainly in the nucleus and is ubiquitously present in various tissues, particularly in ovary, lymph node, endometrium, thyroid, and brain tissue. It plays a significant role in embryonic development, hematopoiesis, and neurodevelopment. Mutations in KMT2A have been associated with several pathological conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KMT2A/MLL1 antibody 29278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KMT2A/MLL1 Polyclonal antibody (Cat# 29278-1-AP) has 3 citations published in Nature Communications, Cancer Letters, and Journal of Inflammation (London). The research fields span epigenetic regulation in pancreatic cancer stem cells, PARP1-mediated tumorigenesis, and histone posttranslational modification in neutrophil plasticity during inflammation. All citations involve human samples with applications including WB, ChIP, and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics | ||
Cancer Lett PARP1 interacts with WDR5 to enhance target gene recognition and facilitate tumorigenesis | ||
J Inflamm (Lond) Histone H3 posttranslational modified enzymes defined neutrophil plasticity and their vulnerability to IL-10 in the course of the inflammation |
