MLLT3/AF9 Polyclonal antibody

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  WB analysis of Jurkat using 12825-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12825-1-AP (MLLT3/AF9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12825-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12825-1-AP (MLLT3/AF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12825-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12825-1-AP (MLLT3/AF9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

MLLT3/AF9 Polyclonal Antibody for WB, ELISA

Cat No. 12825-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AF9, FLJ2035, MLLT3, Protein AF 9, YEATS3

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Tested Applications

• Positive WB detected in: Jurkat cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12825-1-AP targets MLLT3/AF9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3528

  Product name: Recombinant human MLLT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-348 aa of BC036089

  Sequence: MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPPYKVEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGGDPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSGSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKDSREHKSAFKEPSRDHNKSSKESSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKPDSNLLTITSGQDKKAPSKRPPISDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKK

• Full Name: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
• Calculated Molecular Weight: 568 aa, 63 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC036089
• Gene Symbol: AF9/MLLT3
• Gene ID (NCBI): 4300
• RRID: AB_2145949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42568
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLLT3, also named as AF9, YEATS3, is a 568 amino acid protein, which is a component of the superelongation complex6 and co-operates with DOT1L, which di/trimethylates H3K79 to promote transcription. MLLT3 localizes to active transcription start sites (TSSs) through the YEATS domain, which recognizes active histone marks such as H3K9 acetylation and crotonylation. A truncated MLLT3 that lacks the YEATS domain forms a leukaemic fusion protein with the N terminus of MLL1, which misdirects MLLT3-interacting complexes to induce aberrant gene transcription. MLLT3 also regulates erythroid or megakaryocytic progenitors and was identified as a definitive HSC hub gene during mouse development. Researcher found that the expression of a gene called MLLT3 was closely correlated with blood stem cells'potential to self-renew and that the protein generated by the MLLT3 gene provides blood stem cells with the instructions necessary to maintain its ability to self-renew. It does this by working with other regulatory proteins to keep important parts of the blood stem cell's machinery operational as the cells divide.