AF-6 Polyclonal antibody

• 

  WB analysis of mouse kidney using 55102-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55102-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55102-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

AF-6 Polyclonal Antibody for WB, ELISA

Cat No. 55102-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AF 6, AF6, AFADIN, Afadin variant, MLLT4

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Tested Applications

• Positive WB detected in: mouse kidney tissue, A431 cells, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

55102-1-AP targets AF-6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
• Calculated Molecular Weight: 207 kDa
• Observed Molecular Weight: 182-210 kDa
• GenBank Accession Number: NM_005936
• Gene Symbol: Afadin/AF6
• Gene ID (NCBI): 4301
• RRID: AB_10792809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55196
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLLT4, also named as AF6 and Afadin, belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). It is a nNectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. The antibody is specific to MLLT4.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Ethnopharmacol; Wuzi-Yanzong-Wan prevents oligoasthenospermia due to TAp73 suppression by affecting cellular junction remodeling in testicular tissue in mice; Authors - Deling Wu
rat	WB	Res Sq; Novel Populations of Lung Capillary Endothelial Cells and Their Functional Significance; Authors - Joel James
mouse	WB	Aging (Albany NY); The mechanism of simultaneous intake of Jujuboside A and B in the regulation of sleep at the hypothalamic level; Authors - Wei Wang