MCF7 cells were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
MCF7 cells were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20292-1-AP
WB result of MLN64 antibody (20292-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLN64 transfected Jurkat cells.
WB result of MLN64 antibody (20292-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLN64 transfected Jurkat cells.
WB analysis using 20292-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 20292-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 20292-1-AP
IP result of anti-MLN64 (IP:20292-1-AP, 3ug; Detection:20292-1-AP 1:500) with MCF-7 cells lysate 1200ug.
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of MCF-7 using 20292-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20292-1-AP (MLN64 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20292-1-AP (MLN64 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MLN64 (also known as STARD3) is an integral membrane protein anchored in late endosomes. MLN64 has a StAR-related lipid-transfer (START) domain to bind cholesterol, mediating intracellular cholesterol transfer. MLN64 is widely expressed in multiple tissues including liver, spleen, heart, kidney, lung, and the brain. Higher expression of MLN64 has been found in several malignancies. MLN64 can be detected as 33 kDa truncated fragment (PMID:9237999, PMID:15718238).
MCF7 cells were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20292-1-AP
WB result of MLN64 antibody (20292-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLN64 transfected Jurkat cells.
WB analysis using 20292-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 20292-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary tumor using 20292-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary tumor using 20292-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 40x lens).
IP Figures
IP experiment of MCF-7 using 20292-1-AP
IP result of anti-MLN64 (IP:20292-1-AP, 3ug; Detection:20292-1-AP 1:500) with MCF-7 cells lysate 1200ug.
IF/ICC Figures
IF Staining of MCF-7 using 20292-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20292-1-AP (MLN64 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
MLN64 Polyclonal antibody
Catalog Number
20292-1-AP
Citations
3
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
