MLN64 Polyclonal antibody

• Featured Product
• KD/KO Validated

MLN64 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20292-1-AP

Synonyms

CAB1, MLN 64, MLN64, Protein CAB1, STARD3

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Validation Data Gallery

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  WB analysis of Jurkat using 20292-1-AP

  WB result of MLN64 antibody (20292-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLN64 transfected Jurkat cells.

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  WB analysis of MCF-7 using 20292-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20292-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 20292-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 20292-1-AP

  IP result of anti-MLN64 (IP:20292-1-AP, 3ug; Detection:20292-1-AP 1:500) with MCF-7 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 20292-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 20292-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 20292-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20292-1-AP (MLN64 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, mouse placenta tissue, MCF-7 cells, A549 cells, Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 20292-1-AP is MLN64 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLN64 fusion protein Ag14061
• Full Name: StAR-related lipid transfer (START) domain containing 3
• Calculated Molecular Weight: 445 aa, 51 kDa
• Observed Molecular Weight: 33-53 kDa
• GenBank Accession Number: BC008747
• Gene Symbol: MLN64/STARD3
• Gene ID (NCBI): 10948
• RRID: AB_10643978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLN64 (also known as STARD3) is an integral membrane protein anchored in late endosomes. MLN64 has a StAR-related lipid-transfer (START) domain to bind cholesterol, mediating intracellular cholesterol transfer. MLN64 is widely expressed in multiple tissues including liver, spleen, heart, kidney, lung, and the brain. Higher expression of MLN64 has been found in several malignancies. MLN64 can be detected as 33 kDa truncated fragment (PMID:9237999, PMID:15718238).

Publications

Species	Application	Title
human	WB	Autophagy; PMID: 24905460; Authors - Rui Liu
human,mouse	WB,IHC,IF,IP	Life Sci; Blockade of STARD3-mediated cholesterol transport alleviates diabetes-induced podocyte injury by reducing mitochondrial cholesterol accumulation; Authors - Jijia Hu ; KD Validated