Tested Applications
| Positive WB detected in | mouse thymus tissue, mouse ovary tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12042-1-AP targets MLX in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2671 Product name: Recombinant human MLX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC010689 Sequence: MTEPGASPEDPWVKVEYAYSDNSLDPGLFVESTRKGSVVSRANSIGSTSASSVPNTDDEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDNPHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVLHQLKNQLY Predict reactive species |
| Full Name | MAX-like protein X |
| Calculated Molecular Weight | 298 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC010689 |
| Gene Symbol | MLX |
| Gene ID (NCBI) | 6945 |
| RRID | AB_10667462 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UH92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MLX antibody 12042-1-AP | Download protocol |
| WB protocol for MLX antibody 12042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MLX Polyclonal antibody (Cat# 12042-1-AP) has a total of 4 citations appearing in Cell Death & Disease, PLoS Biology, Molecular & Cellular Proteomics, and EMBO Reports. The associated research fields include cancer biology (osteosarcoma and redox homeostasis), reproductive biology (spermatogenesis and metabolic stress), proteomics (NEDD8-activating enzyme inhibition), and RNA biology (lncRNA-mediated translational regulation of the DHX36 helicase).
| Species | Application | Title |
|---|---|---|
Cell Death Dis Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma | ||
PLoS Biol The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.
| ||
Mol Cell Proteomics Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. | ||
EMBO Rep SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase. |
Reviews
What customers say
The sole review for 12042-1-AP is a 5-star rating from Athi Kannan (June 2023), who used the antibody for immunofluorescence on mouse uterine cells. The protocol involved EDTA pH9 antigen retrieval, primary antibody incubation at 1:50 dilution overnight at 4°C, and detection with Alexa Fluor 488 donkey anti-rabbit secondary antibody. The reviewer reported successful results with this workflow.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Athi (Verified Customer) (06-07-2023) | Antigen Retrieval done with EDTA pH9. Primary antibody incubation @4c overnight. Alexa flour 488 donkey anti rabbit secondary antibody is used
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