MLX Polyclonal antibody

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  WB analysis of mouse thymus using 12042-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12042-1-AP (MLX antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 12042-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12042-1-AP (MLX antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12042-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12042-1-AP (MLX antibody) at dilution of 1:50 (under 10x lens).

• Featured Product
• KD/KO Validated

MLX Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12042-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4

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Tested Applications

• Positive WB detected in: mouse thymus tissue, mouse ovary tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12042-1-AP targets MLX in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2671

  Product name: Recombinant human MLX protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-244 aa of BC010689

  Sequence: MTEPGASPEDPWVKVEYAYSDNSLDPGLFVESTRKGSVVSRANSIGSTSASSVPNTDDEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDNPHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVLHQLKNQLY

• Full Name: MAX-like protein X
• Calculated Molecular Weight: 298 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC010689
• Gene Symbol: MLX
• Gene ID (NCBI): 6945
• RRID: AB_10667462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UH92
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IHC	Cell Death Dis; Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma; Authors - Weitang Guo
mouse	WB,IF	EMBO Rep; SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase.; Authors - Julie Martone
human	WB	Mol Cell Proteomics; Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.; Authors - Hua Liao
mouse	IF	PLoS Biol; The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.; Authors - Patrick A Carroll ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Athi (Verified Customer) (06-07-2023)

Antigen Retrieval done with EDTA pH9. Primary antibody incubation @4c overnight. Alexa flour 488 donkey anti rabbit secondary antibody is used

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse uterine cells