Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human renal cell carcinoma tissue, human liver cancer tissue, human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 10302-1-AP is MME,CD10 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MME,CD10 fusion protein Ag0427|
|Full Name||membrane metallo-endopeptidase|
|Calculated molecular weight||750 aa, 86 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC101658|
|Gene ID (NCBI)||4311|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Luca (Verified Customer) (06-10-2022)
I unfortunately could not detect a band against this protein even when expressed using a plasmid, but I have to better optimize my conditions. I liked having the facts sheet with me as that was helpful, so thank you for including it with the Ab.
Boyan (Verified Customer) (10-27-2021)
This antibody recognised a clean band around 100 kd.