MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (3)

Cat no : 10302-1-AP

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

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Validation Data Gallery

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  WB analysis using 10302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10302-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10302-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (MME,CD10 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (MME,CD10 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human liver cancer using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human hepatocirrhosis using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10302-1-AP (MME,CD10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10302-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10302-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of Ramos using 10302-1-AP

  (blue)1x10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human MME,CD10 (10302-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Jurkat using 10302-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MME,CD10 (10302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, rat kidney tissue
• Positive IHC detected in: human kidney tissue, human renal cell carcinoma tissue, human liver cancer tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Jurkat cells, Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10302-1-AP targets MME,CD10 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MME,CD10 fusion protein Ag0427
• Full Name: membrane metallo-endopeptidase
• Calculated Molecular Weight: 750 aa, 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC101658
• Gene Symbol: MME
• Gene ID (NCBI): 4311
• RRID: AB_10640893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Publications

Species	Application	Title
mouse	WB	J Pathol; Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth - SYDE1 regulates placental cell migration and invasion.; Authors - Hsiao-Fan Lo
human,hamster	WB	Fitoterapia; A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ42 production via activating autophagy; Authors - Saijuan Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Luca (Verified Customer) (09-12-2022)

The antibody unfortunately did not work but most likely the conditions of the WB need optimized.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Luca (Verified Customer) (06-10-2022)

I unfortunately could not detect a band against this protein even when expressed using a plasmid, but I have to better optimize my conditions. I liked having the facts sheet with me as that was helpful, so thank you for including it with the Ab.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500- 1:1000

FH

Boyan (Verified Customer) (10-27-2021)

This antibody recognised a clean band around 100 kd.

• Applications: Western Blot