MME,CD10 Polyclonal antibody
MME,CD10 Polyclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC
Conjugate
Unconjugated
Cat no : 23898-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, human kidney tissue, rat kidney tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse kidney tissue, HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 23898-1-AP is MME,CD10 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MME,CD10 fusion protein Ag20963 |
Full Name | membrane metallo-endopeptidase |
Calculated molecular weight | 750 aa, 86 kDa |
Observed molecular weight | 90-110 kDa |
GenBank accession number | BC101658 |
Gene symbol | MME |
Gene ID (NCBI) | 4311 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).
Protocols
Product Specific Protocols | |
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WB protocol for MME,CD10 antibody 23898-1-AP | Download protocol |
IHC protocol for MME,CD10 antibody 23898-1-AP | Download protocol |
IF protocol for MME,CD10 antibody 23898-1-AP | Download protocol |
FC protocol for MME,CD10 antibody 23898-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochem Cell Biol IL-6 induced enhanced clearance of proANP and ANP by insulin-degrading enzyme in T1DM mouse. |