MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

Publications

2

Cat no : 23898-1-AP

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

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Validation Data Gallery

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  WB analysis of mouse kidney using 23898-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 23898-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 23898-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23898-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 23898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 23898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 23898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MME,CD10 antibody (23898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 23898-1-AP

  Immunofluorescent analysis of HepG2 cells, using MME antibody 23898-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 23898-1-AP

  1X10^6 HepG2 cells were stained with .2ug MME,CD10 antibody (23898-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, rat kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse kidney tissue, HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 23898-1-AP is MME,CD10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MME,CD10 fusion protein Ag20963
• Full Name: membrane metallo-endopeptidase
• Calculated molecular weight: 750 aa, 86 kDa
• Observed molecular weight: 90-110 kDa
• GenBank accession number: BC101658
• Gene symbol: MME
• Gene ID (NCBI): 4311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Publications

Species	Application	Title
human	IF	Cell Physiol Biochem; NR4A1 is Involved in Fibrogenesis in Ovarian Endometriosis.; Authors - Xinliu Zeng
mouse	WB	Biochem Cell Biol; IL-6 induced enhanced clearance of proANP and ANP by insulin-degrading enzyme in T1DM mouse.; Authors - Caiyun Li