mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 23898-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 23898-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
human kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 23898-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 23898-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 23898-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 23898-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Neprilysin/CD10 antibody (23898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Neprilysin/CD10 antibody (23898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of Ramos using 23898-1-AP
Immunofluorescent analysis of (4% PFA) fixed Ramos cells using MME,CD10 antibody (23898-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed Ramos cells using MME,CD10 antibody (23898-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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mouse kidney tissue, human kidney tissue, rat kidney tissue
Positive IHC detected in
human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse kidney tissue
Positive IF/ICC detected in
Ramos cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).
Protocols
Product Specific Protocols
WB protocol for Neprilysin/CD10 antibody 23898-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 23898-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 23898-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 23898-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human kidney using 23898-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 23898-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse kidney using 23898-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Neprilysin/CD10 antibody (23898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF/ICC Figures
IF Staining of Ramos using 23898-1-AP
Immunofluorescent analysis of (4% PFA) fixed Ramos cells using MME,CD10 antibody (23898-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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