MME,CD10 Monoclonal antibody

• Featured Product
• KD/KO Validated

MME,CD10 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4B9D9

Cat no : 60034-3-Ig

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 60034-3-Ig

  WB result of MME,CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MME,CD10 transfected HEK-293 cells.

• 

  WB analysis of pig kidney using 60034-3-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 60034-3-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (MME,CD10 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (MME,CD10 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  FC experiment of Ramos using 60034-3-Ig

  1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (60034-3-Ig, Clone:4B9D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Raji using 60034-3-Ig

  1X10^6 Raji cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (60034-3-Ig, Clone:4B9D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: pig kidney tissue, mouse kidney tissue, HEK-293 cells
• Positive IHC detected in: human renal cell carcinoma tissue, human kidney tissue, human tonsil tissue, mouse kidney tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Ramos cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Product Information

60034-3-Ig targets MME,CD10 in WB, IHC, FC, ELISA applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MME,CD10 fusion protein Ag0427
• Full Name: membrane metallo-endopeptidase
• Calculated molecular weight: 750 aa, 86 kDa
• Observed molecular weight: 100 kDa
• GenBank accession number: BC101658
• Gene symbol: MME
• Gene ID (NCBI): 4311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C