MMP17 Polyclonal antibody

MMP17 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28429-1-AP

Synonyms

Matrix metalloproteinase 17, MMP 17, MMP17, MT MMP 4, MT4 MMP, MT4MMP, MTMMP4

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Validation Data Gallery

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  WB analysis using 28429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28429-1-AP (MMP17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 28429-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28429-1-AP (MMP17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28429-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28429-1-AP (MMP17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

28429-1-AP targets MMP17 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP17 fusion protein Ag29298
• Full Name: matrix metallopeptidase 17 (membrane-inserted)
• Calculated Molecular Weight: 67 kDa
• GenBank Accession Number: NM_016155
• Gene Symbol: MMP17
• Gene ID (NCBI): 4326
• RRID: AB_2881140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Transl Med; Prognostic risk factors of serous ovarian carcinoma based on mesenchymal stem cell phenotype and guidance for therapeutic efficacy; Authors - Xiaohui Yang
human	WB	Eur J Pharmacol; Doxycycline hydrochloride inhibits the progress of malignant rhabdoid tumor of kidney by targeting MMP17 and MMP1 through PI3K-Akt signaling pathway; Authors - Tao Mi
human	WB	Biomedicines; CCZ1 Accelerates the Progression of Cervical Squamous Cell Carcinoma by Promoting MMP2/MMP17 Expression; Authors - Jing Yu