MMP28 Polyclonal antibody

• Featured Product
• KD/KO Validated

MMP28 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 18237-1-AP

Synonyms

EPILYSIN, matrix metallopeptidase 28, Matrix metalloproteinase 28, MM28, MMP 28, MMP28

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Validation Data Gallery

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  WB analysis of A549 using 18237-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18237-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 18237-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin cancer using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 18237-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 18237-1-AP (MMP28 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, human colon tissue, mouse heart tissue
• Positive IHC detected in: human kidney tissue, human ovary tissue, human skin cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

18237-1-AP targets MMP28 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP28 fusion protein Ag12980
• Full Name: matrix metallopeptidase 28
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC002631
• Gene Symbol: MMP28
• Gene ID (NCBI): 79148
• RRID: AB_2146444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.

Publications

Species	Application	Title
mouse	IHC,IF	Cell Mol Life Sci; Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons.; Authors - Kyoung-In Cho
human	WB	FASEB J; Drastic induction of MMP-7 by cortisol in the human amnion: implications for membrane rupture at parturition.; Authors - Lu-Yao Wang
human	WB	Am J Pathol; CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2.; Authors - Zhang Min M
mouse	WB,IF	Dis Model Mech; Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.; Authors - Kyoung-In Cho
human	IHC	Am J Pathol; Matrix Metalloproteinase-28 Is a Key Contributor to Emphysema Pathogenesis.; Authors - Anne M Manicone
mouse	IF	JBMR Plus; Preexisting Type 1 Diabetes Mellitus Blunts the Development of Posttraumatic Osteoarthritis.; Authors - Naiomy D Rios-Arce