MMS19 Polyclonal antibody

• Featured Product
• KD/KO Validated

MMS19 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 16015-1-AP

Synonyms

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

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Validation Data Gallery

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  WB analysis using 16015-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16015-1-AP (MMS19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16015-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16015-1-AP (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16015-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16015-1-AP (MMS19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, PC-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 17 publications below
• CoIP: See 1 publications below

Product Information

16015-1-AP targets MMS19 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMS19 fusion protein Ag8792
• Full Name: MMS19 nucleotide excision repair homolog (S. cerevisiae)
• Calculated Molecular Weight: 1030 aa, 113 kDa
• Observed Molecular Weight: 103-113 kDa
• GenBank Accession Number: BC006575
• Gene Symbol: MMS19
• Gene ID (NCBI): 64210
• RRID: AB_2145043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMS19 plays a critical role in the biogenesis of multiple Fe-S proteins, which functions in genomic stability, RNA polymerase II function, and telomere length regulation. MMS19 has a alanine- and leucine-rich N terminus. It possible has a role in nucleotide excision repair(NER) and RNA polymerase II(POL II) transcription by interacting with ERCC2/XPB helicases, both subunits of NER-transcription factor TFIIH. Also MMS19 involves in the regulation of ER. It can function in chromosome segregation by acting as a part of the mitotic spindle-associated MMXD complex.

Publications

Species	Application	Title
human	WB	Cell; Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.; Authors - Tslil Ast
human	WB	Mol Cell; Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.; Authors - Jenny L Weon ; KD Validated
human	WB	Cell Metab; Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.; Authors - Stehling Oliver O ; KD Validated
mouse	WB	Nat Commun; Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases.; Authors - Seung Wook Yang
human	WB	Cell Rep; The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.; Authors - Diana C Odermatt ; KD Validated
mouse	WB	Mol Metab; Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue.; Authors - Colin J Palmer