MNAT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MNAT1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11719-1-AP

Synonyms

CAP35, CDK7/cyclin H assembly factor, Cyclin G1 interacting protein, MAT1, Menage a trois, MNAT1, p35, p36, RING finger protein 66, RING finger protein MAT1, RNF66, TFB3

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Validation Data Gallery

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  WB analysis using 11719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11719-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11719-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11719-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11719-1-AP

  IP result of anti-MNAT1 (IP:11719-1-AP, 4ug; Detection:11719-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of A375 using 11719-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using MNAT1 antibody (11719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 11719-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MNAT1 antibody (11719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A375 cells, MCF-7 cells, NIH/3T3 cells, A431 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: A375 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

11719-1-AP targets MNAT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MNAT1 fusion protein Ag2293
• Full Name: menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)
• Calculated Molecular Weight: 309 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000820
• Gene Symbol: MNAT1
• Gene ID (NCBI): 4331
• RRID: AB_2145054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Int J Biol Sci; Therapeutic Targeting of CDK7 Suppresses Tumor Progression in Intrahepatic Cholangiocarcinoma.; Authors - Hua-Dong Chen
human	WB,IHC	BMC Cancer; MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway.; Authors - Chensheng Qiu ; KD Validated
mouse	WB	Acta Histochem; Cyclin-dependent kinase 5 regulates MAPK/ERK signaling in the skin of mice.; Authors - Xuexian Liu
mouse	WB	Genes Dev; TRF1 uses a noncanonical function of TFIIH to promote telomere replication; Authors - Zhe Yang