MOAP1 Polyclonal antibody

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  WB analysis using 15325-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15325-1-AP (MOAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15325-1-AP (MOAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15325-1-AP (MOAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MOAP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15325-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MAP 1, MAP1, MOAP1, modulator of apoptosis 1, Paraneoplastic antigen Ma4, PNMA4

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

15325-1-AP targets MOAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2876

  Product name: Recombinant human MOAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC015044

  Sequence: MTLRLLEDWCRGMDMNPRKALLIAGISQSCSVAEIEEALQAGLAPLGEYRLLGRMFRRDENRKVALVGLTAETSHALVPKEIPGKGGIWRVIFKPPDPDNTFLSRLNEFLAGEGMTVGELSRALGHENGSLDPEQGMIPEMWAPMLAQALEALQPALQCLKYKKLRVFSGRESPEPGEEEFGRWMFHTTQMIKAWQVPDVEKRRRLLESLRGPALDVIRVLKINNPLITVDECLQALEEVFGVTDNPRELQVKYLTTYQKDEEKLSAYVLRLEPLLQKLVQRGAIERDAVNQARLDQVIAGAVHKTIRRELNLPEDGPAPGFLQLLVLIKDYEAAEEEEALLQAILEGNFT

• Full Name: modulator of apoptosis 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC015044
• Gene Symbol: MOAP1
• Gene ID (NCBI): 64112
• RRID: AB_3085459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96BY2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Modulator of apoptosis 1 (MOAP1, also known as MAP-1 and PNMA4) belongs to the PNMA family and is expressed in various types of tissues (PMID: 36724555). MAP1 contains a central BH3-like motif and interacts with the BAX protein (PMID: 11060313). It plays a central role in mitochondria and death receptor-mediated apoptosis and is associated with many diseases such as cancers and neurological diseases (PMID: 35269511).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Res Sq; The Retrotransposon-Derived Capsid Genes PNMA1 and PNMA4 Maintain Reproductive Capacity; Authors - Luke Berchowitz
mouse	WB	bioRxiv; The retrotransposon - derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity; Authors - Thomas W P Wood ; KO Validated