MOBKL1A Polyclonal antibody

MOBKL1A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12790-1-AP

Synonyms

MATS2, Mob1 homolog 1A, Mob1A, Mob1B, MOB4A, MOBKL1A, Protein Mob4A

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 12790-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12790-1-AP (MOBKL1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12790-1-AP

  IP result of anti-MOBKL1A (IP:12790-1-AP, 4ug; Detection:12790-1-AP 1:1000) with Jurkat cells lysate 2800ug.

• 

  IHC staining of human pancreas cancer using 12790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12790-1-AP (MOBKL1A antibody) at dilution of 1:100 (under 10x lens).

---

Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

12790-1-AP targets MOBKL1A in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOBKL1A fusion protein Ag3509
• Full Name: MOB1, Mps One Binder kinase activator-like 1A (yeast)
• Calculated Molecular Weight: 216 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC038112
• Gene Symbol: MOBKL1A
• Gene ID (NCBI): 92597
• RRID: AB_2266566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Breast Cancer Res; Circular RNA circCCDC85A inhibits breast cancer progression via acting as a miR-550a-5p sponge to enhance MOB1A expression; Authors - Lingjiao Meng
mouse	IHC	Biol Reprod; Lats1 Deletion Causes Increased Germ Cell Apoptosis and Follicular Cysts in Mouse Ovaries.; Authors - Tianyanxin Sun
human	WB	Biochem Genet; Growth Inhibition Accompanied by MOB1 Upregulation in Human Acute Lymphoid Leukemia Cells by 3-Deazaneplanocin A.; Authors - Jianzhen Shen
human	IHC	Cell Death Dis; Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.; Authors - Yang Han