MOCOS Polyclonal antibody

MOCOS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16395-1-AP

Synonyms

Molybdenum cofactor sulfurtransferase, Molybdenum cofactor sulfurase, MoCo sulfurase, MCS, hMCS

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Validation Data Gallery

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  WB analysis of HepG2 using 16395-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16395-1-AP (MOCOS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16395-1-AP

  IP result of anti-MOCOS (IP:16395-1-AP, 4ug; Detection:16395-1-AP 1:300) with HepG2 cells lysate 4000ug.

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  IHC staining of human liver using 16395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16395-1-AP (MOCOS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16395-1-AP (MOCOS antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16395-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MOCOS antibody (16395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16395-1-AP targets MOCOS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOCOS fusion protein Ag9487
• Full Name: molybdenum cofactor sulfurase
• Calculated Molecular Weight: 888 aa, 98 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC012079
• Gene Symbol: MOCOS
• Gene ID (NCBI): 55034
• RRID: AB_2297842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOCOS(Molybdenum cofactor sulfurase) is also named as MCS, MOS, hMCS and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. It catalyzes the removal of elemental sulfur from cysteine to produce alanine. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form. Defects in MOCOS are the cause of xanthinuria type 2 (XU2)(PMID:17368066).