MOF Polyclonal antibody

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• MOF Polyclonal antibody (13842-1-AP)

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• KD/KO Validated

MOF Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 13842-1-AP Copied!

Publications (17)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ChIP, ELISA

Synonyms

MYST1, EC:2.3.1.-, EC:2.3.1.48, Histone acetyltransferase KAT8, hMOF

• 

  WB analysis of HeLa using 13842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 13842-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 13842-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 13842-1-AP

  IP result of anti-MOF (IP:13842-1-AP, 4ug; Detection:13842-1-AP 1:600) with HeLa cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HCT 116 using 13842-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using MOF antibody (13842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, rat liver tissue, mouse liver tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13842-1-AP targets MOF in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, silkworm
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4802

  Product name: Recombinant human MOF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 118-467 aa of BC037773

  Sequence: AVQKNSEKYLSELAEQPERKITRNQKRKHDEINHVQKTYAEMDPTTAALEKEHEAITKVKYVDKIHIGNYEIDAWYFSPFPEDYGKQPKLWLCEYCLKYMKYEKSYRFHLGQCQWRQPPGKEIYRKSNISVYEVDGKDHKIYCQNLCLLAKLFLDHKTLYFDVEPFVFYILTEVDRQGAHIVGYFSKEKESPDGNNVACILTLPPYQRRGYGKFLIAFSYELSKLESTVGSPEKPLSDLGKLSYRSYWSWVLLEILRDFRGTLSIKDLSQMTSITQNDIISTLQSLNMVKYWKGQHVICVTPKLVEEHLKSAQYKKPPITGGWGAAVCRGRWGSVSMWTGRSQGLLIAVT

• Full Name: MYST histone acetyltransferase 1
• Calculated Molecular Weight: 467 aa, 53 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC037773
• Gene Symbol: KAT8/MOF
• Gene ID (NCBI): 84148
• RRID: AB_2146894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H7Z6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOF, also known as MYST1, KAT8, is a member of the MYST acetyltransferase family, which is homologous to Drosophila males absent on the first (MOF) protein. Deletion of MYST1 in cells leads to genomic instability, spontaneous chromosomal aberration, cell cycle defects, reduced transcription of certain genes and defective DNA damage repair (PMID: 32711345). MYST1 dually localizes to the nucleus and mitochondria of human cancer cells (PMID: 37813994). Western blot analysis detected MOF at an apparent molecular mass of 52 kDa.

Publications

What published studies show

The MOF Polyclonal antibody (Cat# 13842-1-AP) has 17 citations across journals including Nature, Autophagy, Nature Communications, Cell Discovery, Redox Biology, Advanced Science, Cell Reports, JCI Insight, Molecular & Cellular Proteomics, FASEB Journal, and Stem Cells. Research fields span copper signaling and inflammation, autophagy/MTORC1 regulation, histone acetylation, chemoresistance, viral replication, rheumatoid arthritis, embryonic development, colitis, lung cancer, oral squamous cell carcinoma, and stem cell reprogramming.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
Mouse	WB	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
human	WB	Cell Discov; Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance; Authors - Yan He
human	WB,ChIP	Redox Biol; Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.; Authors - Ming Kong ; KD Validated
human	WB	Adv Sci (Weinh); Targeting Intratumoral Copper Inhibits Tumor Progression via p62-Mediated EZH2 Degradation and Potentiates Anti-PD-1 Immunotherapy in Oral Squamous Cell Carcinoma; Authors - Xiaohu Lin