MOF Polyclonal antibody

• Featured Product
• KD/KO Validated

MOF Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 13842-1-AP

Synonyms

MYST1, Lysine acetyltransferase 8, KAT8, hMOF, Histone acetyltransferase KAT8

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Validation Data Gallery

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  WB analysis of HeLa using 13842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13842-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13842-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13842-1-AP

  IP result of anti-MOF (IP:13842-1-AP, 4ug; Detection:13842-1-AP 1:600) with HeLa cells lysate 1200ug.

• 

  IF Staining of HCT 116 using 13842-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using MOF antibody (13842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

13842-1-AP targets MOF in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOF fusion protein Ag4802
• Full Name: MYST histone acetyltransferase 1
• Calculated Molecular Weight: 467 aa, 53 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC037773
• Gene Symbol: KAT8/MOF
• Gene ID (NCBI): 84148
• RRID: AB_2146894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOF, also known as MYST1, KAT8, is a member of the MYST acetyltransferase family, which is homologous to Drosophila males absent on the first (MOF) protein. Deletion of MYST1 in cells leads to genomic instability, spontaneous chromosomal aberration, cell cycle defects, reduced transcription of certain genes and defective DNA damage repair (PMID: 32711345). MYST1 dually localizes to the nucleus and mitochondria of human cancer cells (PMID: 37813994). Western blot analysis detected MOF at an apparent molecular mass of 52 kDa.

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB,ChIP	Redox Biol; Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.; Authors - Ming Kong ; KD Validated
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
human	WB	Mol Cell Proteomics; Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.; Authors - Ding Wang
mouse	WB	Cell Physiol Biochem; Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis.; Authors - Yang Yang ; KO Validated