MOGAT2 Polyclonal antibody

MOGAT2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21286-1-AP

Synonyms

DC5, DGAT2L5, hDC5, hMGAT2, MGAT2, MOGAT2

---

Validation Data Gallery

• 

  WB analysis of HL-60 using 21286-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21286-1-AP (MOGAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21286-1-AP (MOGAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 21286-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21286-1-AP (MOGAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21286-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21286-1-AP (MOGAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 21286-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21286-1-AP (MOGAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 21286-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21286-1-AP (MOGAT2 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human kidney tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21286-1-AP is MOGAT2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOGAT2 fusion protein Ag15806
• Full Name: monoacylglycerol O-acyltransferase 2
• Calculated Molecular Weight: 334 aa, 38 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC103878
• Gene Symbol: MOGAT2
• Gene ID (NCBI): 80168
• RRID: AB_10733350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.