MON1A Polyclonal antibody

MON1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 23772-1-AP

Synonyms

MON1 homolog A (yeast), MON1A, SAND1

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Validation Data Gallery

• 

  WB analysis using 23772-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 23772-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23772-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, Jurkat cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 23772-1-AP is MON1A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MON1A fusion protein Ag6900
• Full Name: MON1 homolog A (yeast)
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa and 45 kDa
• GenBank Accession Number: BC047022
• Gene Symbol: MON1A
• Gene ID (NCBI): 84315
• RRID: AB_2879320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MON1A is a cytosolic protein that peripherally associates with the ER. MON1A plays an important role in membrane trafficking through the secretory apparatus. MON1A affects different steps in the secretory pathway including endoplasmic reticulum-to-Golgi traffic. MON1A encodes several isoforms with MW aboout 73, 62, 55, 45 and 34 kDa.

Publications

Species	Application	Title
	WB	Cell Death Dis; TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.; Authors - Jia Hu
human		Autophagy; GORASP2 promotes phagophore closure and autophagosome maturation into autolysosomes; Authors - Yusheng Xing

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Q (Verified Customer) (06-01-2021)

It can produce a stronger band a little higher than 55. But its identity is not quite sure as in human cells Mon1A is supposed to be around 73 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293 cell lysate

MON1A Antibody Western Blot validation (1:1000 dilution) in HEK293 cell lysate (Cat no:23772-1-AP)