MORC3 Polyclonal antibody

MORC3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24994-1-AP

Synonyms

ZCWCC3, ZCW5, NXP2, Nuclear matrix protein 2, MORC family CW-type zinc finger protein 3

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Validation Data Gallery

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  WB analysis of HeLa using 24994-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24994-1-AP (MORC3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 24994-1-AP

  IP result of anti-MORC3 (IP:24994-1-AP, 4ug; Detection:24994-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IF Staining of A431 using 24994-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MORC3 antibody (24994-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

24994-1-AP targets MORC3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MORC3 fusion protein Ag21825
• Full Name: MORC family CW-type zinc finger 3
• Calculated Molecular Weight: 939 aa, 107 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC132731
• Gene Symbol: MORC3
• Gene ID (NCBI): 23515
• RRID: AB_2879836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MORC3, also named as MORC family CW-type zinc finger protein 3 or NXP2, is a 939 amino acid protein, which contains 1 CW-type zinc finger. MORC3 localizes in the nucleus and are expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. MORC3 as Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. Sumoylated MORC3-NBs can also associate with PML-NBs and recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity. It may be required for influenza A transcription during viral infection. The calculated molecular weight of MORC3 is a 107 kDa, but the Sumoylated MORC3 protein is about 130 kDa.

Publications

Species	Application	Title
human	WB	Nature; Self-guarding of MORC3 enables virulence factor-triggered immunity.; Authors - Moritz M Gaidt
human	WB,IF	J Med Virol; MORC3 restricts human cytomegalovirus infection by suppressing the major immediate-early promoter activity.; Authors - Dong Li
mouse	WB	iScience; The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis; Authors - Ziqi Wang