MORF4L1 Polyclonal antibody

MORF4L1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55257-1-AP

Synonyms

Eaf3, FWP006, HSPC008, HSPC061, HsT17725, MORF related gene 15 protein, MORF4L1, MORFRG15, mortality factor 4 like 1, MRG15, PP368, Protein MSL3 1, S863 6

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Validation Data Gallery

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  WB analysis of HEK-293 using 55257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55257-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55257-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55257-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55257-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 55257-1-AP

  IP result of anti-MORF4L1 (IP:55257-1-AP, 4ug; Detection:55257-1-AP 1:800) with HEK-293 cells lysate 1200ug.

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  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HEK-293 using 55257-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human skin tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55257-1-AP targets MORF4L1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mortality factor 4 like 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: NM_006791
• Gene Symbol: MORF4L1
• Gene ID (NCBI): 10933
• RRID: AB_10836938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MORF4L1, also named as MRG15, belongs to the MRG family. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. It is also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. (PMID:14966270) This antibody has no cross-reaction to MORF4L2.